High-resolution melting analysis

High resolution melt technique for detection of mutations, polymorphisms in Dna

High Resolution Melt (HRM) analysis is a powerful technique in molecular biology for the detection of mutations, polymorphisms and epigenetic differences in double-stranded DNA samples. It was discovered and developed by Idaho Technology and the University of Utah. It has advantages over other genotyping technologies, namely:It is cost-effective vs. other genotyping technologies such as sequencing and TaqMan SNP typing. This makes it ideal for large scale genotyping projects. It is fast and powerful thus able to accurately genotype many samples rapidly. It is simple. With a good quality HRM assay, powerful genotyping can be performed by non-geneticists in any laboratory with access to an HRM capable real-time PCR machine.

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