Keywords

#klenow_fragment

Klenow fragment

The Klenow fragment is a large protein fragment produced when DNA polymerase I from E. coli is enzymatically cleaved by the protease subtilisin. First reported in 1970, it retains the 5' → 3' polymerase activity and the 3’ → 5’ exonuclease activity for removal of precoding nucleotides and proofreading, but loses its 5' → 3' exonuclease activity.

Wed 29th

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Klenow fragment DNA polymerase I Thermostable DNA polymerase Taq polymerase Thermal cycler Proteolysis Variants of PCR History of polymerase chain reaction Loop-mediated isothermal amplification Hi-C (genomic analysis technique)
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