Scanning electron cryomicroscopy

Scanning electron cryomicroscopy (CryoSEM) is a form of electron microscopy where a hydrated but cryogenically fixed sample is imaged on a scanning electron microscope's cold stage in a cryogenic chamber. The cooling is usually achieved with liquid nitrogen. CryoSEM of biological samples with a high moisture content can be done faster with fewer sample preparation steps than conventional SEM. In addition, the dehydration processes needed to prepare a biological sample for a conventional SEM chamber create numerous distortions in the tissue leading to structural artifacts during imaging.

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