Surface plasmon resonance microscopy

Surface plasmon resonance microscopy (SPRM), also called surface plasmon resonance imaging (SPRI), is a label free analytical tool that combines the surface plasmon resonance of metallic surfaces with imaging of the metallic surface. The heterogeneity of the refractive index of the metallic surface imparts high contrast images, caused by the shift in the resonance angle. SPRM can achieve a sub-nanometer thickness sensitivity and lateral resolution achieves values of micrometer scale. SPRM is used to characterize surfaces such as self-assembled monolayers, multilayer films, metal nanoparticles, oligonucleotide arrays, and binding and reduction reactions. Surface plasmon polaritons are surface electromagnetic waves coupled to oscillating free electrons of a metallic surface that propagate along a metal/dielectric interface. Since polaritons are highly sensitive to small changes in the refractive index of the metallic material, it can be used as a biosensing tool that does not require labeling. SPRM measurements can be made in real-time, such as measuring binding kinetics of membrane proteins in single cells, or DNA hybridization.

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